KAPA HyperPrep Kit, PCR-free from Roche

The KAPA Hyper Prep Kit is a versatile, streamlined solution for DNA library preparation

The novel one-tube chemistry, optimally formulated and evolved enzymes enable higher yields of adapter-ligated library and lower amplification bias. This translates to higher library diversity, lower duplication rates and more uniform coverage, particularly for FFPE and low-input samples.

Features:
Improve library yields and sequence quality -

• Higher library yields translate to greater molecular complexity
• Fewer cycles of amplification with KAPA HiFi DNA Polymerase results in lower duplication rates and improved coverage

Create high-quality libraries from FFPE samples -

• Generate high-quality libraries from 250 ng FFPE DNA or less
• Significantly lower duplication rates and higher coverage

Complete library construction in less than 3 hours -

• One-tube workflow with minimal cleanup steps reduces overall time, and minimizes hands-on time
• Sample-to-library in less than 2 hours for pcr-free workflows, or less than 3 hours with amplification
• Fewer handling steps lead to improved consistency and reproducibility

Improve performance with low-input samples -

• Generate more sequence-quality library molecules without increasing adapter-dimers
• Increase yields without inducing size bias